Synthesis of L-nucleic acids by means of an enzyme

US11634741B2 · kind B2 · utility

Assignee

• Aptarion Biotech AG · DE

Inventors

• Andreas Pech · Bodenwerder, DE
• Florian Jarosch · Berlin, DE
• Michael Jahnz · Berlin, DE
• Sven Klussmann · Berlin, DE
• Ralf David · Leipzig, DE

Key dates

• Filing date: Nov 20, 2014
• Grant date: Apr 25, 2023
• Expiry date: Aug 23, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y207/07007
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is related to a method for adding one or more L-nucleotides to the 3′end of a first L-nucleic acid, wherein the method comprises the step of reacting the one or more L-nucleotides with the first L-nucleic acid in the presence of a protein comprising a mutant enzymatic activity exhibiting moiety, wherein the enzymatic activity is capable of adding one or more L-nucleotides to the 3′ end of the first L-nucleic acid, wherein the mutant enzymatic activity exhibiting moiety comprises an amino acid sequence, wherein the amino acids of the amino acid sequence are D-amino acids, wherein the mutant enzymatic activity exhibiting moiety is a variant of an enzymatic activity exhibiting moiety, wherein the enzymatic activity exhibiting moiety consists of an amino acid sequence according to SEQ ID NO: 15 and wherein the amino acids of the amino acid sequence according to SEQ ID NO: 15 are D-amino acids, wherein the amino acid sequence of the mutant enzymatic activity exhibiting moiety differs from the amino acid sequence of the enzymatic activity exhibiting moiety consisting of an amino acid sequence according to SEQ ID NO: 15 at least at one amino acid position, preferably…