MSP nanopores and related methods

US11634764B2 · kind B2 · utility

Assignees

• University of Washington · US
• THE UAB RESEARCH FOUNDATION · US

Inventors

• Jens H. Gundlach · Seattle, US
• Michael Niederweis · Homewood, US
• Thomas Z. Butler · Seattle, US
• Mikhail Pavlenok · Birmingham, US
• Mark A. Troll · Palo Alto, US
• Suja Sukumaran · Dublin, US

Key dates

• Filing date: Dec 14, 2020
• Grant date: Apr 25, 2023
• Expiry date: Dec 14, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N24/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.