Method of determining target nucleic acid
US11634769B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 2018 |
| Grant date | Apr 25, 2023 |
| Priority date | — |
| Expiry date | Jan 19, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/141
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of determining one or more target nucleic acids in cells includes the steps of: delivering one or more probes into the cells, each of the one or more probes being capable of binding with corresponding target nucleic acid present in the cells to form a double-stranded sequence; inserting an array of functionalized nanoneedles into the cells to bind with the double-stranded sequence; and hybridizing the bound double-stranded sequence with a first and second DNA sequence to produce a hybridized product, the first and second DNA sequence being at least partially complementary to each other. A kit for determining a target nucleic acid in cells includes a first reagent comprising a probe for binding with the target nucleic acid to form a double-stranded sequence; and an array of functionalized nanoneedles comprising a protein for binding with the double-stranded sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.