Extracellular vesicle isolation by nanomembranes
US11635357B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 22, 2021 |
| Grant date | Apr 25, 2023 |
| Priority date | — |
| Expiry date | Jul 22, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4088
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.