Method for verifying the primary structure of protein
US11635437B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 23, 2016 |
| Grant date | Apr 25, 2023 |
| Priority date | — |
| Expiry date | Jul 25, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2570/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is a method for verifying the primary structure of a protein through comparative analyses between ion clusters observed in mass spectra and a series of simulated ion clusters deduced from its putative chemical formula. The method comprises the steps of: preparing a protein sample for mass spectrometric analyses; collecting mass spectra of the protein sample; obtaining master ion cluster from a plurality of ion clusters in the mass spectra; producing a series of simulated ion clusters according to the chemical formula of the protein; finding the best fit for the master ion cluster among the series of simulated ion clusters; and verifying if said best-fit simulated ion cluster corresponds to the chemical formula of the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.