Spectroscopic single-molecule localization microscopy
US11635607B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 18, 2021 |
| Grant date | Apr 25, 2023 |
| Priority date | — |
| Expiry date | May 18, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0638
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“−1st”) light using a grating; wherein the 1st light comprises spectral information and the −1st light comprises spectral information; capturing the 1st light and the −1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.