Determining 5′ transcript sequences

US11639517B2 · kind B2 · utility

Assignee

• Becton, Dickinson and Company · US

Inventors

• Christina Chang · Menlo Park, US
• Eleen Shum · San Jose, US

Key dates

• Filing date: Sep 30, 2019
• Grant date: May 2, 2023
• Expiry date: Nov 25, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. There are provided, in some embodiments, methods of 5′-based and 3′-based gene expression profiling. Immune repertoire profiling methods are also provided in some embodiments.