Patent · US Active

Method for selecting biological binding molecules

US11644459B2 · kind B2 · utility

0Cited by

1References

15Claims

0Family size

Assignee

AVA LIFESCIENCE GMBH · DE

Inventors

Holger Klapproth · Glückstadt, DE
Marcus DUHREN-VON MINDEN · Müllheim, DE

Key dates

Filing date	Dec 19, 2019
Grant date	May 9, 2023
Priority date	—
Expiry date	Jan 12, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/00
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention relates to the field of producing, identifying, and selecting biological binding molecules, e.g. in particular antibodies or fragments thereof, which selectively bind to somatically hypermutated B-cell receptors or B-cell receptor complexes. The method is used in order to select a biological binding molecule which specifically binds to a B-cell receptor having hypermutated regions as the target receptor, but not to a B-cell receptor without hypermutated regions, and is carried out in a cell-based system using immature B cells which are in the pro/pre stage and cause a ‘Triple Knockout’ of the genes for RAG2 or RAG1, Lambda5, and SLP65.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.