Genetically modified rabbit expressing an exogenous protein in milk

US11647737B2 · kind B2 · utility

Assignees

• ASC THERAPEUTICS INC. · US
• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US

Inventors

• Ruby Yanru TSAI · Milpitas, US
• Jie Xu · Foster City, US
• Yuqing E. Chen · Ann Arbor, US
• Jifeng Zhang · Newbury Park, US
• Ling-jie KONG · Foster City, US

Key dates

• Filing date: Dec 29, 2017
• Grant date: May 16, 2023
• Expiry date: Feb 24, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a genetically modified non-human mammal that comprises an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein the anchor DNA sequence comprises a site-specific recombinase recognition site. Also provided is a genetically modified non-human mammal that comprises a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein the transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein the transgene is flanked by a pair of site-specific recombinase resulting sites. The genetically modified non-human mammals provided can be used for producing the secreted recombinant protein encoded by the transgene from the milk produced by the genetically modified non-human mammals.