Nucleic acid sequencing method and nucleic acid sequencing kit

US11649489B2 · kind B2 · utility

Assignee

• BGI SHENZHEN · CN

Inventors

• Erkai LIU · Lo Wu, CN
• Wenwei Zhang · Longbeilingcun, CN
• Ao Chen · Lo Wu, CN
• Chongjun Xu · San Jose, US

Key dates

• Filing date: Oct 12, 2018
• Grant date: May 16, 2023
• Expiry date: Oct 11, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6876
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3′ end, a polymerase, a reagent 1 for excising the blocking group attached to the 3′ end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.