Methods and compositions for quantitation of proteins and RNA
US11649497B2 · kind B2 · utility
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186References
23Claims
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Key dates
| Filing date | Jan 12, 2021 |
| Grant date | May 16, 2023 |
| Priority date | — |
| Expiry date | Jul 19, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6804
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein include systems, methods, compositions, and kits for determining expressions of proteins and genes simultaneously, and for sample indexing. The method can comprise extending a cellular component-binding reagent specific oligonucleotide hybridized to an oligonucleotide barcode to generate an extended cellular component-binding reagent specific oligonucleotide. The extended cellular component-binding reagent specific oligonucleotide can be separated from the oligonucleotide barcodes. The separated extended cellular component-binding reagent specific oligonucleotide can be amplified separately from barcoded cDNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.