Systems and methods for genetic identification and analysis

US11655498B2 · kind B2 · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Darrell Ricke · San Diego, US
• James D. Harper · Hazel Green, US
• Brian S. Helfer · Brookline, US
• Joseph Isaacson · Latham, US
• Adam M. Michaleas · Hudson, US
• Martha S. Petrovick · Barre, US
• Eric Schwoebel · Woburn, US
• Anna Shcherbina · East Palo Alto, US
• Philip Fremont-Smith · Medford, US
• James G. Watkins · Sutton, US
• Edward C. Wack · Waltham, US

Key dates

• Filing date: Jul 6, 2018
• Grant date: May 23, 2023
• Expiry date: Nov 6, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure provides various systems and methods for identifying individuals from one or more samples. In particular, improved systems and methods of analysis are provided for handling multiple contributors, as well as systems and methods that model not only individual error rates per locus but factor in amplification of errors induced through PCR cycles. In some embodiments, modeling of error rates can be applied in multi-contributor settings to more accurately establish real alleles from artifacts. Other aspects involve application of sequencing in error modeling. Further, methods are provided for determining the presence of common individual DNA profiles in one or more complex DNA mixtures and for deconvolution of multiple complex DNA mixtures into shared individual components.