DNA aptamer-cyanine complexes as mephedrone and cannabinoid colorimetric sensors
US11656235B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 7, 2021 |
| Grant date | May 23, 2023 |
| Priority date | — |
| Expiry date | Aug 7, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/948
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The subject invention provides materials and methods for single-step detection of target molecules in a sample. The methods and assays of the subject invention employ a dye-displacement strategy, in which aptamers complexed with a cyanine dye for sensitive and rapid detection of targets of interest. In the presence of a target, aptamer-target binding liberates the non-covalently bound aptamer-binding dye, resulting in optical changes that can be observed spectrophotometrically or with the naked eye. The methods and assays of the subject invention enable the colorimetric detection of targets of interest regardless of their structure, sequence, target-binding affinity, and physicochemical properties of their targets.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.