Mutant of lycopene epsilon cyclase (LCYE) gene crucial in wheat carotenoid synthesis pathway and use thereof

US11661594B2 · kind B2 · utility

Assignee

• Crop Research Institute, Shandong Academy of Agricultural Sciences · CN

Inventors

• Shengnan ZHAI · Shandong, CN
• Jianjun Liu · Aurora, US
• Haosheng Li · Shandong, CN
• Jianmin Song · Shandong, CN
• Aifeng Liu · Shandong, CN
• Xinyou Cao · Shandong, CN
• Dungong Cheng · Shandong, CN
• Zhendong Zhao · Nanjing, CN
• Cheng LIU · Shandong, CN
• Jun Guo · Fremont, US
• Ran HAN · Shandong, CN
• Yan ZI · Shandong, CN
• Faji LI · Shandong, CN
• Xiaolu Wang · Beijing, CN

Key dates

• Filing date: Mar 8, 2021
• Grant date: May 30, 2023
• Expiry date: Jul 16, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y505/01018
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure discloses a mutant of a lycopene epsilon cyclase (Lcye) gene crucial in a wheat carotenoid synthesis pathway and use thereof. The present disclosure provides the following proteins: (1) a protein obtained by substituting serine at position 253 of an Lcye-D1 protein with phenylalanine; (2) a derived protein that is obtained by subjecting the protein in (1) to substitution and/or deletion and/or addition of one or more amino acid residues and has the same ability as the protein in (1); (3) a protein that has a homology of more than 99%, more than 95%, more than 90%, more than 85%, or more than 80% with the amino acid sequence defined in any one of (1) and (2) and has the same function as the amino acid sequence; and (4) a fusion protein obtained by attaching a tag to N-terminus and/or C-terminus of the protein in any one of (1) to (3). The present disclosure not only verifies the function of an Lcye gene, but also provides a theoretical basis and a germplasm resource for improving the color character of flour and products thereof.