Oligonucleotides associated with antibodies

US11661631B2 · kind B2 · utility

Assignee

• Becton, Dickinson and Company · US

Inventors

• Eleen Shum · San Jose, US
• Katherine Lazaruk · Redwood City, US
• Dennis Prosen · San Jose, US
• Devon Jensen · San Jose, US
• David Rosenfeld · Brentwood, US
• Janice Hoiyi Lai · Mountain View, US
• James Ghadiali · San Diego, US
• Christina Chang · Menlo Park, US

Key dates

• Filing date: Jan 21, 2020
• Grant date: May 30, 2023
• Expiry date: Jan 30, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/185
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).