Multilayered cell sheet of neural crest stem cells and method of preparing the same
US11666684B2 · kind B2 · utility
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Key dates
| Filing date | Dec 18, 2017 |
| Grant date | Jun 6, 2023 |
| Priority date | — |
| Expiry date | May 7, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of manufacturing a multilayered cell sheet of neural crest stem cells (NCSCs), includes: (1) isolating and culturing NCSCs from peripheral nerves; (2) embedding the cultured NCSCs in a hydrogel; (3) culturing the hydrogel comprising the NCSCs embedded therein under stressed culture conditions in which a physical support is applied; and (4) culturing the resulting hydrogel of step (3) under non-stressed culture conditions in which a physical support is removed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.