Method of producing a recombinant protein
US11667887B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | May 13, 2016 |
| Grant date | Jun 6, 2023 |
| Priority date | — |
| Expiry date | Jul 19, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/569
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method of producing a recombinant protein in a host cell comprising adding Polyethyleneimine (PEI) during cell culture. Addition of PEI to the cell culture as a fermentation enhancer can result in reducing the viscosity of the cell culture, and/or increasing the extracellular concentration of the recombinant protein, and/or reducing the duration of cell culture to the point of harvest or protein recovery.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.