Methods and compositions for identifying ligands on arrays using indexes and barcodes

US11667957B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Darren Segale · San Diego, US
• Fiona E. Black · San Diego, US
• Jeffrey Brodin · Evanston, US
• Jeffrey S. Fisher · San Francisco, US
• Lorenzo Berti · San Diego, US
• Siew Hong Leong · Singapore, SG

Key dates

• Filing date: Oct 23, 2019
• Grant date: Jun 6, 2023
• Expiry date: Apr 5, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6816
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. In some embodiments, the barcode and the index are determined by sequencing.