Products and processes for multiplex nucleic acid identification

US11667958B2 · kind B2 · utility

Assignee

• AGENA BIOSCIENCE, INC. · US

Inventors

• Christiane Honisch · Village of La Jolla, US
• Dirk Johannes Van Den Boom · Dreieich, DE
• Michael Mosko · Poway, US
• Anders Olof Herman Nygren · San Diego, US

Key dates

• Filing date: Feb 18, 2020
• Grant date: Jun 6, 2023
• Expiry date: Feb 18, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6858
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.