Method for determining the level of zygosity of a seed
US11668703B2 · kind B2 · utility
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12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 23, 2018 |
| Grant date | Jun 6, 2023 |
| Priority date | — |
| Expiry date | Mar 29, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method for determining the level of transgene zygosity in a poaceae seed, the transgene being genetically linked to a gene coding for a fluorescent protein (FP protein) under the control of a promoter operative in the endosperm, comprising the step of exposing the endosperm of the seed to a wavelength exciting the FP protein, and measuring the intensity of the emitted fluorescence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.