Continuous, solvent-free and non-enzymatic peptide synthesis by reactive extrusion

US11673912B2 · kind B2 · utility

Assignees

• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR
• Universite De Montpellier · FR
• Ecole Nationale Supérieure de Chimie de Montpellier · FR
• ECOLE DES MINES D'ALES · FR
• Association Pour La Recherche Developpement Des Methodes Et Processus Industriels · FR

Inventors

• Yves Yeboue · Montpellier, FR
• Benjamin Gallard · Sarcelles, FR
• Nicolas Le Moigne · Saint-Sébastien-d'Aigrefeuille, FR
• Frédéric Lamaty · Montpellier, FR
• Jean Martinez · Caux, FR
• Thomas-Xavier Metro · Montpellier, FR

Key dates

• Filing date: Feb 12, 2019
• Grant date: Jun 13, 2023
• Expiry date: Mar 16, 2039

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B29K2089/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A continuous, solvent-free and non-enzymatic method for synthesizing a compound of formula (I): Ra-POLYPEP-Rc (I) wherein: POLYPEP is a poly-amino acid compound, Ra and Rc are as specified, the method including the steps of: a) feeding an extrusion reactor with (1) a compound of formula (II) Ra-PEPNt-Rg (II) wherein; PEPNt is a mono- or a poly-amino acid compound, Ra and Rg are as specified, and (2) a compound of formula (III) H-PEPCt-Rc (III) wherein: PEPCt is a mono- or a poly-amino acid compound, and Rc is as defined in the absence of any solvent, so that the compound of formula (II) and the compound of formula (III) react together for generating a compound of formula (I), and b) collecting the compound of formula (I) from the extrusion reactor.