In vivo de-glycosylation of recombinant proteins by co-expression with PNGase F
US11673926B2 · kind B2 · utility
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Key dates
| Filing date | Jun 7, 2012 |
| Grant date | Jun 13, 2023 |
| Priority date | — |
| Expiry date | May 4, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8242
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Materials and methods for in vivo de-glycosylation of recombinant N-glycosylated proteins by co-expression with bacterial PNGase F (Peptide: N-glycosidase F) in plants, using a transient expression system are described. Methods are described which, for example, produce recombinant proteins of interest in plants in a non-glycosylated form. A method of expressing active bacterial PNGase F in plants also is provided.
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