Methods for producing mutant bacteriophages for the detection of listeria
US11674124B2 · kind B2 · utility
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29Claims
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Key dates
| Filing date | Jun 18, 2020 |
| Grant date | Jun 13, 2023 |
| Priority date | — |
| Expiry date | Oct 1, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for the production of mutant bacteriophages with altered host range. Additionally, disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.