Method of isolating nucleic acids and the system thereof
US11674134B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 7, 2021 |
| Grant date | Jun 13, 2023 |
| Priority date | — |
| Expiry date | Aug 16, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1013
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention discloses a method of isolating nucleic acid and the system thereof, which relates to the following process: (1) providing a biological sample containing cells; (2) performing a cell concentration procedure; (3) performing a magnetic separation procedure through a magnetic separation unit; (4) performing a suspension procedure through a suspending unit; (5) performing a lysis procedure through a lysis unit; and (6) performing a nucleic acid extraction procedure to extract the nucleic acids from the biological samples; wherein the cell concentration procedure does not involve any means of centrifugation. Therefore, the method is simple and efficient, and it is particularly favorable in full automation of nucleic acid isolation. Furthermore, the method and the system as a whole further include a clarification procedure executed by a clarification unit without using centrifugation means, so that improves the quality of nucleic acid isolation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.