Selective advantage in fermentation

US11674162B2 · kind B2 · utility

Assignee

• Ginkgo Bioworks, Inc. · US

Inventors

• Emily H. Greenhagen · Melrose, US
• Maureen Hamilton · Littleton, US
• William G. LaTouf · Belmont, US
• Andrew L. Consiglio · Holden, US
• Kyle M. MacEwen · Braintree, US
• Arthur J. Shaw, IV · Belmont, US

Key dates

• Filing date: Apr 8, 2015
• Grant date: Jun 13, 2023
• Expiry date: Apr 8, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y305/99004
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are transformed cells and related nucleotide and protein sequences, and fermentation compositions and methods, all of which are related to providing selective advantage in fermentation. For example, a selective advantage results from transformation of a cell with a nucleic acid that allows a transformed cell to metabolize one or more nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell cannot metabolize, and from fermentation of the transformed cell using one or more feedstocks, such as fractioned grain, which are depleted in or free of conventional nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell can metabolize. Also disclosed are methods for improved oxygen transfer in an aerobic or microaerobic fermentation.