Hooked probe, method for ligating nucleic acid and method for constructing sequencing library

US11680285B2 · kind B2 · utility

Assignee

• MGI TEGH GO., LTD. · CN

Inventors

• Yuan Jiang · Richland, US
• Yang Xi · 安丰镇, CN
• Wenwei Zhang · Longbeilingcun, CN
• Pengjuan LIU · Longbeilingcun, CN
• Xia Zhao · Highland Park, US
• Qiaoling Li · Beijing, CN
• Hanjie SHEN · Lo Wu, CN
• Yongwei Zhang · Beijing, CN
• Radoje Drmanac · Los Altos Hills, US

Key dates

• Filing date: Nov 9, 2017
• Grant date: Jun 20, 2023
• Expiry date: Jun 18, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6876
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.