Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US11680293B1 · kind B1 · utility
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24Claims
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Key dates
| Filing date | Apr 21, 2022 |
| Grant date | Jun 20, 2023 |
| Priority date | — |
| Expiry date | Apr 21, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, apparatuses and compositions for generating highly sensitive and accurate sequencing results of massive parallel sequencing (NGS). The methods and compositions may be referred to as SubDivision-Seq, and may comprise two parts. The first part includes making a target-enriched DNA library, organizing the UMIs on DNA molecules to form primary clones and subdividing the primary clones into subclones. The second part includes sequencing the DNA library by NGS, deducing consensus sequence from each subclone, then deducing consensus sequence in each primary clone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.