Stable water isotope labeling and magnetic resonance imaging for visualization of the presence of and prediction of the likelihood of occurence of rapidly dividing cells

US11684306B2 · kind B2 · utility

Assignee

• THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES et al. · US

Inventors

• Nataliya Buxbaum · Bethesda, US
• Donald Farthing · Bethesda, US
• Martin Lizak · Bethesda, US
• Helmut Merkle · Bethesda, US
• Natella Maglakelidze · Bethesda, US
• Brittany Alexandrea Oliver · Bethesda, US
• Ronald Gress · Gaithersburg, US

Key dates

• Filing date: Oct 27, 2017
• Grant date: Jun 27, 2023
• Expiry date: May 23, 2040

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61B2503/40
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure generally relates to stable water isotope labeling followed by detection via MRI (swiMRI), including deuterium MRI (dMRI) and 17O MRI, for visualizing rapidly dividing immune cells within target and/or lymphoid organ/s and/or tissues affected by chronic graft-versus-host disease (cGVHD). Using deuterated water labeling, followed by dMRI, a distinction in deuterium signal was detected in a target organ (e.g. liver) of the cGVHD-affected mice compared to unaffected mice, i.e. syngeneic HSCT recipient mice, where the host and donor are matched, and normal (unmanipulated) mice.