Nanofiber-based long-term primary hepatocyte three-dimensional culture system and culturing method
US11685900B2 · kind B2 · utility
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Key dates
| Filing date | Sep 21, 2018 |
| Grant date | Jun 27, 2023 |
| Priority date | — |
| Expiry date | Oct 31, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/70
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a nanofiber-based long-term primary hepatocyte culture system and a culture method, wherein the primary hepatocyte culture system has an advantage that it can culture cells in three-dimensions in vitro to maintain the original physiological activity of low proliferative primary hepatocytes for a long time by co-culturing indirectly by separating primary hepatocytes and hepatic non-parenchymal cells with a support consisting of nanofibers therebetween without direct co-culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.