Method of producing thin enzyme-based sensing layers on planar sensors

US11690544B2 · kind B2 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Samson CHEN · Pasadena, US
• Axel Scherer · Laguna Beach, US
• Dvin Adalian · Pasadena, US
• Peter A. Petillo · Lawrence, US
• Muhammad Musab Jilani · Pasadena, US
• Xiomara L. Madero · Glendale, US
• Deepan Kishore Kumar · San Jose, US

Key dates

• Filing date: May 7, 2021
• Grant date: Jul 4, 2023
• Expiry date: Jan 7, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y101/03041
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.