Snake venom thrombin-like enzyme marker peptide of Agkistrodon halys pallas and its application in the species identification of hemocoagulase for injection
US11692186B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 30, 2021 |
| Grant date | Jul 4, 2023 |
| Priority date | — |
| Expiry date | Jul 30, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2560/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a snake venom thrombin marker peptide of Agkistrodon Halys Pallas and an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme of Agkistrodon Halys Pallas and improves the quality control level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.