Small molecule biosensors

US11693017B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• Daniel J. Mandell · Brookline, US
• Justin Feng · Cambridge, US
• Xavier Rios Villanueva · Boston, US
• Rajagopal Chari · Watertown, US

Key dates

• Filing date: Jan 12, 2016
• Grant date: Jul 4, 2023
• Expiry date: Dec 5, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Biosensors for small molecules can be used in applications that range from metabolic engineering to orthogonal control of transcription. Biosensors are produced based on a ligand-binding domain (LBD) using a method that, in principle, can be applied for any target molecule. The LBD is fused to either a fluorescent protein or a transcriptional activator and is destabilized by mutation such that the fusion accumulates only in cells containing the target ligand. The power of this method is illustrated by developing biosensors for digoxin and progesterone. Addition of ligand to cells expressing a biosensor activates transcription in yeast, mammalian cells and plants, with a dynamic range of up to about 100-fold or more. The biosensors are used to improve the biotransformation of pregnenolone to progesterone in yeast and to regulate CRISPR activity in mammalian cells. This work provides a general methodology to develop biosensors for a broad range of molecules.