Patent · US Active

Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing

US11697843B2 · kind B2 · utility

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226References
20Claims
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Assignee

Inventors

Key dates

Filing dateMay 14, 2021
Grant dateJul 11, 2023
Priority date—
Expiry dateJul 3, 2041

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6855
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Provided herein are methods, compositions and kits for the generation of bisulfite-converted next generation sequencing (NGS) libraries. The methods, compositions and kits provided herein can be useful, for example, for the production of libraries from genomic DNA that allow for determination of the methylation status across the genome, i.e. the methylome. The methods, compositions and kits provided herein can also be utilized to query methylation status at a particular genomic locus or loci. Moreover, the methods provided herein can be employed for high-throughput sequencing of bisulfite-converted DNA while maintaining the directional (strandedness) information of the original nucleic acid sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.