Reagent and method for fluorescence quantitative real-time PCR detection of RCL
US11697848B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2019 |
| Grant date | Jul 11, 2023 |
| Priority date | — |
| Expiry date | Jul 29, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a reagent and method for detecting a replication-competent lentivirus (RCL) by fluorescence quantitative real-time polymerase chain reaction (PCR). In particular, the present invention provides a primer and probe combination for detecting RCL, and a method for performing detection using said primer and probe; the present invention also provides a reagent kit comprising said primer and probe. The primer and probe combination of the present invention detects RCL with high amplification efficiency and good specificity, and can be used for RCL detection and RCL monitoring of clinical patient peripheral blood samples which may occur during a production process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.