Method and system for analysing fluorospot assays

US11698342B2 · kind B2 · utility

Assignee

• MABTECH AB · SE

Inventors

• Christian Smedman · Stockholm, SE
• Joakim Jalden · Sollentuna, SE
• Daniel Pelikan · Vänge, SE
• Pol Del Aguila Pla · Solna, SE
• Klas Magnusson · Stuvsta-Snättringe, SE

Key dates

• Filing date: Jun 27, 2018
• Grant date: Jul 11, 2023
• Expiry date: Feb 8, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6471
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed is a method for analysing FluoroSpot assays. The method comprises illuminating a well of an assay plate with at least one excitation light, capturing at least one image of the well, in raw image format, for each excitation light, generating a model of analyte release distribution in the well for each excitation light, and clustering a plurality of co-positioned fluorospots as a multiple secretion fluorospot, wherein the clustering is performed for all generated models, and wherein the clustering determines at least one multiple secretion fluorospot. The generation of the model of analyte release distribution for a given excitation light comprises deconvolving the captured at least one image of the well to estimate a pre-diffusion analyte distribution, and detecting potential analyte release sites based on local maxima therein.