In vitro methods of differentiating stem cells into neurons and neurons generated using the same

US11702630B2 · kind B2 · utility

Assignees

• The J. David Gladstone Institute, a testamentary trust established under the Will of J. David Gladstone · US
• The Regents of the University of California · US
• Georgia Tech Research Corporation · US

Inventors

• Jessica Butts · San Francisco, US
• Todd Christopher McDevitt · San Francisco, US

Key dates

• Filing date: May 26, 2017
• Grant date: Jul 18, 2023
• Expiry date: Dec 30, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/90
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods of generating spinal cord glutamatergic interneurons (V2a interneurons) from human pluripotent stem cells (hPSCs) are provided. A method of the present disclosure may include culturing a first population of hPSCs in vitro in a neural induction medium that includes: a retinoic acid signaling pathway activator; a sonic hedgehog (Shh) signaling pathway activator; and a Notch signaling pathway inhibitor, wherein the culturing results in generation of a second population of cultured cells containing CHX10+ V2a interneurons. Also provided are non-human animal models that include the hPSC-derived spinal cord glutamatergic interneurons, and methods of producing the non-human animal models.