Method of storing a separation matrix

US11708390B2 · kind B2 · utility

Assignee

• Cytiva BioProcess R&D AB · SE

Inventors

• Annika Forss · Uppsala, SE
• Gustav Rodrigo · Uppsala, SE
• Tomas Bjorkman · Uppsala, SE
• Mats Ander · Uppsala, SE
• Jesper Ulf Hansson · Uppsala, SE

Key dates

• Filing date: May 10, 2017
• Grant date: Jul 25, 2023
• Expiry date: Aug 11, 2040

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2220/52
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days. The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.