Method, system and recombinant bacmid for preparation of recombinant adeno-associated virus

US11708585B2 · kind B2 · utility

Assignee

• WUHAN INSTITUTE OF PHYSICS AND MATHEMATICS, CHINESE ACADEMY OF SCIENCES · CN

Inventors

• Yang-Chang Wu
• Fuqiang XU · Shanghai, CN
• Ting Mei · 红钢城街道, CN
• Liangyu JIANG · 红钢城街道, CN
• Zengpeng Han · 红钢城街道, CN

Key dates

• Filing date: Jun 28, 2019
• Grant date: Jul 25, 2023
• Expiry date: Oct 16, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14151
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of preparing a recombinant adeno-associated virus (rAAV) including: (1) preparing a shuttle plasmid and a corresponding recombinant bacmid including a baculovirus genome, where the shuttle plasmid includes at least an rAAV gene of interest flanked by inverted terminal repeats (ITR-GOI) integrated with a heterologous functional gene fragment, and the recombinant bacmid includes an expression cassette of functional protein components necessary for assembly of the rAAV; (2) integrating the rAAV ITR-GOI and the expression cassette of functional protein components by using the shuttle plasmid and the recombinant bacmid, to yield a recombinant bacmid including a recombinant baculovirus genome; and (3) transfecting, with the recombinant bacmid, a host cell line.