Microfluidic pH-stat for point of care (POC) enzyme diagnostics
US11709146B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 3, 2019 |
| Grant date | Jul 25, 2023 |
| Priority date | — |
| Expiry date | Dec 28, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/84
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A microfluidic pH-stat with a specially-is designed slide and portable device can be used for point-of-care enzyme diagnostics. The slide includes a microchamber and a substrate for the enzyme being tested. The substrate is homogenized with the sample in the microchamber to form a test volume. The microchamber includes a working microelectrode that injects current to split water in the test volume to generate hydrogen ions and/or hydroxide ions and a micro-pH-electrode to measure a pH of the test volume; the slide also includes a reference microelectrode. The device includes a processor to adjust the injected current based on the pH of the test volume and determine an activity of the enzyme based on an amount the injected current is adjusted.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.