Enhanced selection of efficient targeted genome manipulating agents
US11713455B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 2020 |
| Grant date | Aug 1, 2023 |
| Priority date | — |
| Expiry date | Nov 21, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
For enhanced selection of efficient targeted genome manipulating agents, an apparatus includes first and second chip-based biosensors having one or more sensing surfaces configured to detect biomolecular binding interactions between a nucleic acid sample and a targeted genome manipulating agent functionalized to a capture surface within a sensing range of the one or more sensing surfaces. The first chip-based biosensor uses a nucleic acid sample incubated with a blocking agent that blocks on-target binding and the second chip-based biosensor holds a nucleic acid sample that omits the blocking agent. A measurement apparatus measures first and second sets of response signals produced in response to the biomolecular binding interactions occurring between the nucleic acid sample and the targeted genome manipulating agent. An analysis module determines the genome manipulating efficiency parameters of the targeted genome manipulating agent. A system and a method perform the functions of the apparatus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.