Mapping the location, type and strand of damaged and/or mismatched nucleotides in double-stranded DNA
US11713484B2 · kind B2 · utility
0Cited by
14References
34Claims
0Family size
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Key dates
| Filing date | Aug 21, 2018 |
| Grant date | Aug 1, 2023 |
| Priority date | — |
| Expiry date | Jun 6, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/117
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.
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