Patent · US Active

Hybridization chain reaction methods for in situ molecular detection

US11713485B2 · kind B2 · utility

2Cited by
111References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 3, 2021
Grant dateAug 1, 2023
Priority date—
Expiry dateAug 3, 2041

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2320/10
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.