Time-based cluster imaging of amplified contiguity-preserved library fragments of genomic DNA

US11714848B2 · kind B2 · utility

Assignee

• Illumina Cambridge Limited · GB

Inventors

• Natalie Morrell · Cambridge, GB
• Andrew Slatter · London, GB
• Vicki Thomson · Cambridge, GB

Key dates

• Filing date: Dec 1, 2020
• Grant date: Aug 1, 2023
• Expiry date: Dec 1, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2304/13
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.