Method for improving one-time seedling rate of microspore embryoids of brassica campestris SSP. chinensis makino

US11716944B1 · kind B1 · utility

Assignees

• Anhui Agricultural University · CN
• Anhui Wanjiang Vegetable Industry Technology Research Institute Co., Ltd. · CN

Inventors

• Lingyun Yuan · Hefei, CN
• Liting Zhang · Yongchun County, CN
• Chenggang Wang · Zhejiang, CN
• Xingxue Huang · Hefei, CN
• Shidong Zhu · Hefei, CN
• Jinlong Zhang · 红钢城街道, CN
• Jinfeng Hou · Hefei, CN
• Xiaoyan Tang · Hefei, CN
• Zegen Zhang · Hefei, CN

Key dates

• Filing date: Sep 20, 2022
• Grant date: Aug 8, 2023
• Expiry date: Sep 20, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N5/04
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

The present invention provides a method for improving a one-time seedling rate of microspore embryoids of Brassica campestris ssp. Chinensis Makino, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl2; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4° C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25° C. and 14 h of illumination by blue-red compound light/day until seedlings.