Nanoparticle-based lipase biosensor utilizing a custom-synthesized peptidyl-ester substrate

US11718868B2 · kind B2 · utility

Assignee

• The Government of the United States of America, as represented by the Secretary of the Navy · US

Inventors

• Igor L. Medintz · Springfield, US
• Joyce Breger · Greenbelt, US
• Kimihiro Susumu · Alexandria, US
• Sebastian A. Diaz · Washington, US
• Jesper Brask · Jægersborg, DK

Key dates

• Filing date: Jan 29, 2021
• Grant date: Aug 8, 2023
• Expiry date: Dec 16, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/924
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Lipase activity can be detected with a biosensor that includes a quantum dot adhered to a construct having a lipase-cleavable ester to attach a fluorophore acceptor configured as a Förster resonance energy transfer (FRET) acceptor to the QD when the construct is bound thereto. Cleavage of the ester by a lipase results in a measurable reduction in FRET. In further embodiments, the cleavable ester can be used to detect esterase activity, or the ester could be replaced with a glycosidic linkage to detect glycoside activity.