Patent · US Active

Hybridization chain reaction methods for in situ molecular detection

US11718874B2 · kind B2 · utility

2Cited by

102References

16Claims

0Family size

Assignee

President and Fellows of Harvard College · US

Inventors

Evan R. Daugharthy · Cambridge, US
George M. Church · Brookline, US

Key dates

Filing date	Oct 25, 2018
Grant date	Aug 8, 2023
Priority date	—
Expiry date	Oct 25, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2320/10
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.