Selective degradation of wild-type DNA and enrichment of mutant alleles using nuclease
US11725230B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Jun 24, 2016 |
| Grant date | Aug 15, 2023 |
| Priority date | — |
| Expiry date | Nov 12, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6883
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods for preparing a target mutant nucleic acid for subsequent enrichment relative to a wild type nucleic acid using nucleases that have a substantially higher activity on double stranded DNA versus single stranded DNA or RNA. The present disclosure also includes methods for enriching a target mutant nucleic acid and for preparing unmethylated/methylated nucleic acids of interest for subsequent enrichment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.