Image processing and segmentation of sets of Z-stacked images of three-dimensional biological samples
US11727586B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 2020 |
| Grant date | Aug 15, 2023 |
| Priority date | — |
| Expiry date | Jun 16, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30096
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Methods are provided to project depth-spanning stacks of limited depth-of-field images of a sample into a single image of the sample that can provide in-focus image information about three-dimensional contents of the image. These methods include applying filters to the stacks of images in order to identify pixels within each image that have been captured in focus. These in-focus pixels are then combined to provide the single image of the sample. Filtering of such image stacks can also allow for the determination of depth maps or other geometric information about contents of the sample. Such depth information can also be used to inform segmentation of images of the sample, e.g., by further dividing identified regions that correspond to the contents of the sample at multiple different depths.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.