Tm mapping method

US11732311B2 · kind B2 · utility

Assignee

• National University Corporation University of Toyama · JP

Inventors

• Hideki Niimi · Osaka, JP
• Shinya Otsuki · Nagaokakyo, JP
• Isao Kitajima · Toyama, JP

Key dates

• Filing date: Jun 19, 2020
• Grant date: Aug 22, 2023
• Expiry date: May 23, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The improved Tm mapping method using imperfect-match linear long quenching probes can accurately distinguish among and identify microorganisms at least at the genus level and often at the species level even in a real-time PCR instrument having measurement errors of Tm values between PCR tubes within ±0.5° C. Therefore, the Tm mapping method can be performed in almost all real-time PCR instruments and can identify unspecified infection-causing pathogenic microorganisms in about 4 hours after sample collection.