Rapid diagnostic test for lamp

US11732315B2 · kind B2 · utility

Assignee

• New England Biolabs, Inc. · US

Inventors

• Nathan Tanner · West Newbury, US
• Yinhua Zhang · North Reading, US
• Eric Hunt · Betchworth, GB
• Gregory Patton · Peabody, US
• Guoping Ren · Danvers, US
• Zhiru Li · Lexington, US
• Andrew Barry · Ipswich, US
• Nicole Nichols · Reading, US
• Catherine B. Poole · Mishawaka, US
• Harriet M. Strimpel · Attleboro Falls, US
• Ivan R. Correa, Jr. · Ipswich, US
• Clotilde Carlow · Ipswich, US
• Esta Slayton · Epping, US
• Thomas C. Evans · Somerville, US

Key dates

• Filing date: Sep 28, 2022
• Grant date: Aug 22, 2023
• Expiry date: Sep 28, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/78
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity. The compositions and methods may use any single improvement or combination of improvements selected from thermolabile enzyme variants, poloxamers, various salts, indicators and one or more LAMP primer sets for detecting single and/or multiple targets, probes for detecting variants of the targets including SARS-CoV-2 variants and lateral flow devices.